Post Doc in Molecular Engineering (Technical University of Munich)

PostDoc position in Molecular engineering of a novel type of genetically encoded RNA delivery vehicle 02.02.2023, Wissenschaftliches Personal Within the ‘Chair of Developmental Genetics’, our group combines cutting-edge cell and protein engineering methods for developing novel molecular tools to precisely manipulate the genome and transcriptome of cells and organisms. We apply these tools to various disease models ranging from cell lines and patient-derived stem cells to mouse models. In the past, we used our technologies to treat devasting diseases like Parkinson’s Disease, Duchenne Muscular Dystrophy, or COVID-19 in cellular and animal models. Recently, we developed a synthetic protein to package and transfer RNA into target cell types. With this tool, we were able to express reporter and CRISPR proteins in up to 99% of cells. We now seek to further improve this technology by screening multiple configurations of the transfer protein, by programming target cell types, and by applying the technology to different cellular, ex vivo and in vivo preclinical models. Your Responsibilities: Iterative design, build, test cycle of genetic constructs to express RNA delivery proteins Testing of different cargo RNAs with a focus on CRISPR/Cas enzymes • Exploring of novel approaches for cell type-specific delivery (e.g. by transmembrane protein engineering) Analysis of delivery efficiencies in different cellular models and benchmarking against conventional delivery technologies (LNP, AAVs) • Writing of manuscripts and drafting of scientific illustrations Contribution to patent applications and commercialization strategies Supervision of students (if desired) Your Scientific Qualifications: PhD in Bioengineering / Molecular Biology / Biotechnology / Biochemistry or related fields Good publication record, preferentially with first author publication Theoretical and practical knowledge of advanced DNA cloning techniques, including synthetic genes Experience in mammalian cell cultivation, genetic and/or protein engineering Hands-on experience in cellular assays and analytical techniques (e.g. Luciferase assays, Fluorescence microscopy, Immunostaining, Western Blot, Flow Cytometry, etc.) Knowledge of Genome Editing technologies (e.g. Cas9, Cas13, Base Editing, Prime Editing, CRISPRa/i) is an advantage Your Personal Qualifications: Enthusiasm for solving biological problems by applying engineering principals Drive to tackle and overcome highly challenging scientific problems Deep passion to innovate at the interface of research and clinical translation Commitment to working in a fast-moving and highly competitive field Additional words to describe you: Proactive, curious, creative, team worker Excellent communication skills in written and spoken English Embedded in the flourishing research environment of two world-class universities, and renowned research institutes in the greater Munich area and equipped with state-of-the-art instruments, the Helmholtz Center Munich - at which our laboratory is located - is an outstanding place for conducting research and for career development. Beyond scientific excellence, Munich offers a wide range of leisure activities ranging from numerous lakes to the close vicinity of the alps. Salary is in accordance with the collective agreement for the public sector (federal government). The position is initially limited to two years and should begin as soon as possible. We are looking forward to receiving your application, including a letter of motivation, CV and contact details of at least two references to ‘idg-sekretariat@helmholtz-muenchen.de’. Additionally, we ask you to decide for one of your publications and tell us what it makes so special for you personally. Curious? Don’t hesitate to contact us for additional information. Join us on our mission toward a new era of precise RNA-based medicines! Hinweis zum Datenschutz: Im Rahmen Ihrer Bewerbung um eine Stelle an der Technischen Universität München (TUM) übermitteln Sie personenbezogene Daten. Beachten Sie bitte hierzu unsere Datenschutzhinweise gemäß Art. 13 Datenschutz-Grundverordnung (DSGVO) zur Erhebung und Verarbeitung von personenbezogenen Daten im Rahmen Ihrer Bewerbung. Durch die Übermittlung Ihrer Bewerbung bestätigen Sie, dass Sie die Datenschutzhinweise der TUM zur Kenntnis genommen haben. Kontakt: idg-sekretariat@helmholtz-muenchen.de If you apply for this position please say you saw it on Medicinoxy

PostDoc position in Molecular engineering of a novel type of genetically encoded RNA delivery vehicle

02.02.2023, Wissenschaftliches Personal

Within the ‘Chair of Developmental Genetics’, our group combines cutting-edge cell and protein engineering methods for developing novel molecular tools to precisely manipulate the genome and transcriptome of cells and organisms. We apply these tools to various disease models ranging from cell lines and patient-derived stem cells to mouse models. In the past, we used our technologies to treat devasting diseases like Parkinson’s Disease, Duchenne Muscular Dystrophy, or COVID-19 in cellular and animal models. Recently, we developed a synthetic protein to package and transfer RNA into target cell types. With this tool, we were able to express reporter and CRISPR proteins in up to 99% of cells. We now seek to further improve this technology by screening multiple configurations of the transfer protein, by programming target cell types, and by applying the technology to different cellular, ex vivo and in vivo preclinical models.

Your Responsibilities:

Iterative design, build, test cycle of genetic constructs to express RNA delivery proteins
Testing of different cargo RNAs with a focus on CRISPR/Cas enzymes • Exploring of novel approaches for cell type-specific delivery (e.g. by transmembrane protein engineering)
Analysis of delivery efficiencies in different cellular models and benchmarking against conventional delivery technologies (LNP, AAVs) • Writing of manuscripts and drafting of scientific illustrations
Contribution to patent applications and commercialization strategies
Supervision of students (if desired)

Your Scientific Qualifications:

PhD in Bioengineering / Molecular Biology / Biotechnology / Biochemistry or related fields
Good publication record, preferentially with first author publication
Theoretical and practical knowledge of advanced DNA cloning techniques, including synthetic genes
Experience in mammalian cell cultivation, genetic and/or protein engineering
Hands-on experience in cellular assays and analytical techniques (e.g. Luciferase assays, Fluorescence microscopy, Immunostaining, Western Blot, Flow Cytometry, etc.)
Knowledge of Genome Editing technologies (e.g. Cas9, Cas13, Base Editing, Prime Editing, CRISPRa/i) is an advantage

Your Personal Qualifications:

Enthusiasm for solving biological problems by applying engineering principals
Drive to tackle and overcome highly challenging scientific problems
Deep passion to innovate at the interface of research and clinical translation
Commitment to working in a fast-moving and highly competitive field
Additional words to describe you: Proactive, curious, creative, team worker
Excellent communication skills in written and spoken English

Embedded in the flourishing research environment of two world-class universities, and renowned research institutes in the greater Munich area and equipped with state-of-the-art instruments, the Helmholtz Center Munich - at which our laboratory is located - is an outstanding place for conducting research and for career development. Beyond scientific excellence, Munich offers a wide range of leisure activities ranging from numerous lakes to the close vicinity of the alps.

Salary is in accordance with the collective agreement for the public sector (federal government). The position is initially limited to two years and should begin as soon as possible.

We are looking forward to receiving your application, including a letter of motivation, CV and contact details of at least two references to ‘idg-sekretariat@helmholtz-muenchen.de’. Additionally, we ask you to decide for one of your publications and tell us what it makes so special for you personally.

Curious? Don’t hesitate to contact us for additional information. Join us on our mission toward a new era of precise RNA-based medicines!

Hinweis zum Datenschutz:
Im Rahmen Ihrer Bewerbung um eine Stelle an der Technischen Universität München (TUM) übermitteln Sie personenbezogene Daten. Beachten Sie bitte hierzu unsere Datenschutzhinweise gemäß Art. 13 Datenschutz-Grundverordnung (DSGVO) zur Erhebung und Verarbeitung von personenbezogenen Daten im Rahmen Ihrer Bewerbung. Durch die Übermittlung Ihrer Bewerbung bestätigen Sie, dass Sie die Datenschutzhinweise der TUM zur Kenntnis genommen haben.

Kontakt: idg-sekretariat@helmholtz-muenchen.de

If you apply for this position please say you saw it on Medicinoxy

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