Research Associate in Clinical Sciences (Imperial College London Institute of Clinical Sciences)

We are seeking a research Associate to work in the Sarkies Lab at the Medical Research Council (MRC) London Institute of Medical Sciences, funded by Worldwide Cancer Research. Recently, the Sarkies laboratory has shown that DNA methyltransferases have an off-target effect whereby they introduce a toxic form of DNA damage, 3meC, into DNA. This requires the activity of a specialised repair enzyme, ALKB2, and as a result, DNA... Job listing information Reference MED01663 Date posted 13 January 2020 Closing date 16 February 2020 Key information about the role Location Royal Brompton Campus (map) Position type Full time, fixed term Salary £40,215 plus benefits Department Institute of Clinical Sciences Category Researcher / Non Clinical Researcher Job description Job summary We are seeking a research Associate to work in the Sarkies Lab at the Medical Research Council (MRC) London Institute of Medical Sciences, funded by Worldwide Cancer Research. Recently, the Sarkies laboratory has shown that DNA methyltransferases have an off-target effect whereby they introduce a toxic form of DNA damage, 3meC, into DNA. This requires the activity of a specialised repair enzyme, ALKB2, and as a result, DNA methyltransferases co-evolve with the enzyme ALKB2 across a range of species (Rosic et al., Nature Genetics, 2018). Duties and responsibilities You will explore the implications of this finding for cancer biology. In particular, we aim to test whether the levels and activity of DNA methyltransferases in cancer cells are shaped by the expression of ALKB2, by modulating the levels of both ALKB2 and DNA methyltransferases in cancer cells using CRISPR-Cas9 mediated genome editing. Essential requirements The ideal candidate will have experience in cell culture, ideally using cancer cells, CRISPR-Cas9-mediated genome editing and experience in either DNA damage or epigenetics. You will gain the opportunity to work in a dynamic laboratory at the interface between evolution and epigenetics and will be able to spearhead new applications of this approach to human disease. You will be able to work in a highly collaborative and innovative environment both within the Sarkies lab and across the MRC London Institute of Medical Sciences, a core-funded institute with excellent links to Imperial College London. Further information The post is full time and fixed-term from 01^st May 2020 until 30^th April 2023. Candidates who have not yet been officially awarded their PhD will be appointed as Research Assistant within the salary range £35,477 - £38,566 per annum. For informal enquiries please contact Dr Sonia Nielles-Vallespin (s.nielles-vallespin@imperial.ac.uk), Dr Andrew Scott (a.scott07@imperial.ac.uk) or Professor David Firmin (d.firmin@imperial.ac.uk). For technical issues when applying online please email recruitment@imperial.ac.uk. The College is a proud signatory to the San-Francisco Declaration on Research Assessment (DORA), which means that in hiring and promotion decisions, we evaluate applicants on the quality of their work, not the journal impact factor where it is published. For more information, see https://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-evaluation/ The College believes that the use of animals in research is vital to improve human and animal health and welfare. Animals may only be used in research programmes where their use is shown to be necessary for developing new treatments and making medical advances. Imperial is committed to ensuring that, in cases where this research is deemed essential, all animals in the College’s care are treated with full respect, and that all staff involved with this work show due consideration at every level. http://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-integrity/animal-research/ If you apply for this position please say you saw it on Medicinoxy

We are seeking a research Associate to work in the Sarkies Lab at the Medical Research Council (MRC) London Institute of Medical Sciences, funded by Worldwide Cancer Research. Recently, the Sarkies laboratory has shown that DNA methyltransferases have an off-target effect whereby they introduce a toxic form of DNA damage, 3meC, into DNA. This requires the activity of a specialised repair enzyme, ALKB2, and as a result, DNA...

Job listing information

Reference MED01663
Date posted 13 January 2020
Closing date 16 February 2020

Key information about the role

Location Royal Brompton Campus (map)

Position type Full time, fixed term
Salary £40,215 plus benefits

Department Institute of Clinical Sciences
Category Researcher / Non Clinical Researcher

Job description

Job summary

We are seeking a research Associate to work in the Sarkies Lab at the Medical Research Council (MRC) London Institute of Medical Sciences, funded by Worldwide Cancer Research.

Recently, the Sarkies laboratory has shown that DNA methyltransferases have an off-target effect whereby they introduce a toxic form of DNA damage, 3meC, into DNA. This requires the activity of a specialised repair enzyme, ALKB2, and as a result, DNA methyltransferases co-evolve with the enzyme ALKB2 across a range of species (Rosic et al., Nature Genetics, 2018).

Duties and responsibilities

You will explore the implications of this finding for cancer biology. In particular, we aim to test whether the levels and activity of DNA methyltransferases in cancer cells are shaped by the expression of ALKB2, by modulating the levels of both ALKB2 and DNA methyltransferases in cancer cells using CRISPR-Cas9 mediated genome editing.

Essential requirements

The ideal candidate will have experience in cell culture, ideally using cancer cells, CRISPR-Cas9-mediated genome editing and experience in either DNA damage or epigenetics. You will gain the opportunity to work in a dynamic laboratory at the interface between evolution and epigenetics and will be able to spearhead new applications of this approach to human disease. You will be able to work in a highly collaborative and innovative environment both within the Sarkies lab and across the MRC London Institute of Medical Sciences, a core-funded institute with excellent links to Imperial College London.

Further information

The post is full time and fixed-term from 01^st May 2020 until 30^th April 2023.

Candidates who have not yet been officially awarded their PhD will be appointed as Research Assistant within the salary range £35,477 - £38,566 per annum.

For informal enquiries please contact Dr Sonia Nielles-Vallespin (s.nielles-vallespin@imperial.ac.uk), Dr Andrew Scott (a.scott07@imperial.ac.uk) or Professor David Firmin (d.firmin@imperial.ac.uk).

For technical issues when applying online please email recruitment@imperial.ac.uk.

The College is a proud signatory to the San-Francisco Declaration on Research Assessment (DORA), which means that in hiring and promotion decisions, we evaluate applicants on the quality of their work, not the journal impact factor where it is published. For more information, see https://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-evaluation/

The College believes that the use of animals in research is vital to improve human and animal health and welfare. Animals may only be used in research programmes where their use is shown to be necessary for developing new treatments and making medical advances. Imperial is committed to ensuring that, in cases where this research is deemed essential, all animals in the College’s care are treated with full respect, and that all staff involved with this work show due consideration at every level. http://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-integrity/animal-research/

If you apply for this position please say you saw it on Medicinoxy

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